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cf r d systems 1726 jg recombinant human scf protein r d systems 255 sc recombinant human tgf beta 1 cho expressed  (R&D Systems)


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    R&D Systems cf r d systems 1726 jg recombinant human scf protein r d systems 255 sc recombinant human tgf beta 1 cho expressed
    Cf R D Systems 1726 Jg Recombinant Human Scf Protein R D Systems 255 Sc Recombinant Human Tgf Beta 1 Cho Expressed, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1+scf+r+d+systems+255+sc/Recombinant+Human+Jagged+2+Fc+Chimera+Protein%2C+CF/pm41512836-315-137-138
    Average 94 stars, based on 14 article reviews
    cf r d systems 1726 jg recombinant human scf protein r d systems 255 sc recombinant human tgf beta 1 cho expressed - by Bioz Stars, 2026-09
    94/100 stars

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    Cell Culture:

    Article Title: Targeted Inhibition of ELANE Expression Using Adenine Base Editing to Treat Severe Congenital Neutropenia
    Article Snippet: CD34+ cells were expanded for up to seven days in CD34+ cell expansion medium (StemSpan SFEM II, StemCell Technologies, #09655) supplemented with 1 % penicillin/streptomycin, 1 % Glutamine and cytokines: 20 ng/ml IL-3 (BioLegend, #578006), 20 ng/ml IL-6 (R&D Systems, #7270-IL-100/CF), 20 ng/ml TPO (BioLegend, #763706), 50 ng/ml SCF (R&D Systems, #11010-SC-100) and 50 ng/ml FLT3L (BioLegend, #550606). .. Liquid culture differentiation (LCD) of primary human CD34+ HSPCs CD34+ HSPCs from ELANE-CN patients or HDs were cultured for seven days in differentiation medium 1 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and human recombinant cytokines: 5 ng/ml SCF (R&D Systems, #11010-SC-100), 5 ng/ml IL-3 (BioLegend, #578006), 5 ng/ml GM-CSF (Peprotech, #300-03), 1 ng/ml G-CSF (Lenograstim, Granocyte, Chugai Pharma, PZN 7253193) at a density of > 2x105 cells/ml, followed by seven days in differentiation medium 2 (RPMI supplemented Jo urn al Pr e-p oo f 17 with 10% FCS, 1% penicillin/streptomycin, 1 ng/ml G-CSF (Lenograstim, Granocyte, Chugai Pharma, PZN 7253193). ..

    Article Title: Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE.
    Article Snippet: CD34+ cells were enriched by immunomagnetic bead selection using a CliniMACS Plus or AutoMACS instrument (Miltenyi Biotec). .. Human CD34+ cells were cultured in vitro using complete medium (CM) consisting of StemSpan II (StemCell Technologies) medium supplemented with 100 ng μl−1 human stem cell factor (R&D systems, 255-SC/CF), 100 ng μl−1 human thrombopoietin (R&D systems, 288-TP/CF) and 100 ng μl−1 human FLT-3 ligand (R&D systems, 308-FK/CF). ..

    Article Title: Targeted inhibition of ELANE expression using adenine base editing to treat severe congenital neutropenia
    Article Snippet: CD34 + cells were expanded for up to 7 days in CD34 + cell expansion medium (StemSpan SFEM II, STEMCELL Technologies, #09655) supplemented with 1% penicillin/streptomycin, 1% glutamine, and cytokines: 20 ng/mL IL-3 (BioLegend, #578006), 20 ng/mL IL-6 (R&D Systems, #7270-IL-100/CF), 20 ng/mL TPO (BioLegend, #763706), 50 ng/mL SCF (R&D Systems, #11010-SC-100), and 50 ng/mL FLT3L (BioLegend, #550606). .. CD34 + HSPCs from ELANE -CN patients or HDs were cultured for 7 days in differentiation medium 1 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and human recombinant cytokines: 5 ng/mL SCF [R&D Systems, #11010-SC-100], 5 ng/mL IL-3 [BioLegend, #578006], 5 ng/mL GM-CSF [PeproTech, #300-03], and 1 ng/mL G-CSF [Lenograstim, Granocyte, Chugai Pharma, PZN 7253193]) at a density of >2 × 10 5 cells/mL, followed by 7 days in differentiation medium 2 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and 1 ng/mL G-CSF [Lenograstim, Granocyte, Chugai Pharma, PZN 7253193]). ..

    Recombinant:

    Article Title: Targeted Inhibition of ELANE Expression Using Adenine Base Editing to Treat Severe Congenital Neutropenia
    Article Snippet: CD34+ cells were expanded for up to seven days in CD34+ cell expansion medium (StemSpan SFEM II, StemCell Technologies, #09655) supplemented with 1 % penicillin/streptomycin, 1 % Glutamine and cytokines: 20 ng/ml IL-3 (BioLegend, #578006), 20 ng/ml IL-6 (R&D Systems, #7270-IL-100/CF), 20 ng/ml TPO (BioLegend, #763706), 50 ng/ml SCF (R&D Systems, #11010-SC-100) and 50 ng/ml FLT3L (BioLegend, #550606). .. Liquid culture differentiation (LCD) of primary human CD34+ HSPCs CD34+ HSPCs from ELANE-CN patients or HDs were cultured for seven days in differentiation medium 1 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and human recombinant cytokines: 5 ng/ml SCF (R&D Systems, #11010-SC-100), 5 ng/ml IL-3 (BioLegend, #578006), 5 ng/ml GM-CSF (Peprotech, #300-03), 1 ng/ml G-CSF (Lenograstim, Granocyte, Chugai Pharma, PZN 7253193) at a density of > 2x105 cells/ml, followed by seven days in differentiation medium 2 (RPMI supplemented Jo urn al Pr e-p oo f 17 with 10% FCS, 1% penicillin/streptomycin, 1 ng/ml G-CSF (Lenograstim, Granocyte, Chugai Pharma, PZN 7253193). ..

    Article Title: Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rhesus CD34+ antibody (Clone 12.8) Fred Hutchinson Cancer Research Center N/A CD34-PE antibody (Clone 563) BD Biosciences # 550761 RRID:AB_393871 Mouse anti-human fetal hemoglobin BD Biosciences # 551796 RRID:AB_394253 APC rat anti-mouse IgG1 (Clone X56) BD Biosciences # 550874 RRID:AB_398470 CD14-FITC (Clone M5E2) BD Bioscience # 555397 RRID:AB_395798 CD20-PE (Clone 2H7) BD Bioscience # 555623 RRID:AB_395989 CD34-APC (Clone 563) BD Bioscience # 561209 RRID:AB_10683161 CD45-V450 (Clone D058-1283) BD Bioscience # 561291 RRID:AB_10611576 CD71-PE (Clone DF1513) GeneTex # GTX43030 RRID:AB_11170434 CD45-FITC (Clone MB4-6D6) Miltenyi Biotech # 130-119-764 RRID:AB_2751825 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 CD45RA-VioBlue (Clone T6D11) Miltenyi Biotech # 130-113-360 RRID:AB_2726132 CD117-PE (Clone 104D2) BioLegend # 313204 RRID:AB_314983 Goat anti-human IgG-AF488 Jackson ImmunoResearch # 109-545-088 RRID:AB_2337838 CD45-APC (Non-competing MB4-6D6 clone) Miltenyi Biotech # 130-123-787 RRID:AB_2819522 CD3-BV605 (Clone SP34) BD Bioscience # 562994 RRID:AB_2737938 CD8-APC-Vio 770 (Clone BW135/80) Miltenyi Biotech # 130-113-155 RRID:AB_2725983 CD4-BV650 (Clone L200) BD Bioscience # 563737 RRID:AB_2687486 CD20-BV421 (Clone 2H7) BioLegend # 302330 RRID:AB_10965543 CD45RA-PerCP-Cy5.5 (Clone T6D11) Miltenyi Biotech # 130-113-359 RRID:AB_2751109 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 Anti-mouse IgM beads Miltenyi Biotech # 130-047-302 RRID:AB_244359 Biological samples Frozen healthy human umbilical cord derived CD34+ HSPCs AllCells N/A Frozen/fresh rhesus CD34+ HSPCs NIH N/A Cryopreserved bone marrow CD34+ HSPCs StemCell Technologies # 70002 Chemicals, peptides, and recombinant proteins 7-AAD BioLegend # 420403 Granulocyte-Colony Stimulating Factor (G-CSF) Amgen NDC 55513-0530-10 Plerixafor Sigma-Aldrich # 110078-46-1 Ficoll-Paque PLUS density gradient media GE Healthcare # 17144002 Human stem cell factor (SCF) R&D Systems # 255-SC/CF Human thrombopoietin (TPO) R&D Systems # 288-TP/CF Human recombinant human Flt3-ligand, (Flt3) R&D Systems # 308-TP/CF 33NLS SpCas9 Laboratory of Scot A. Wolfe N/A Interleukin-3 (IL-3) R&D Systems 203-IL-100/CF (Continued on next page) Cell Stem Cell 32, 209–226.e1–e8, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Erythropoietin (EPO) Amgen # 55513-144-10 Dexamethasone VETone # 501012 Depo-estradiol Pfizer NDC 00009-0271-01 Fetal bovine serum (FBS) ThermoFisher Scientific # 43803 FBS Gemini # 100-106 Insulin Lily NDC 00002-8215 Holo-transferrin Sigma Aldrich # T0665-1G Bovine serum albumin (BSA) Roche # 3116956001 TCEP Thermo Fisher Scientific # 20490 Hoechst 33342 Thermo Fisher Scientific # R37165 IL-6 R&D Systems # 206-IL-200/CF IL-2 NCI # Ro 23-6019 RetroNectin (Takara Bio) # T100B Busulfex PDL BioPharma NDC 59148-070-90 Hydroxyurea Bristol-Myers Squibb NDC 68084- 284-01 Critical commercial assays Gibco CTS Detachable Dynabeads CD3/CD28 Kit Thermo Fisher Scientific # A56992 CellTrace Far Red Cell Proliferation Kit Thermo Fisher Scientific # C34564 Deposited data Raw sequencing data This paper PRJNA1045609 Experimental models: Cell lines

    Article Title: Tunable differentiation of human CD4 + and CD8 + T cells from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-human CCR7 BV605 (clone 2-L1-A) BD Biosciences 566754; RRID: AB_2869850 Mouse anti-human CCR7 BUV661 (clone 2-L1-A) BD Biosciences 749824; RRID: AB_2874072 Mouse anti-human CXCR3 BV421 (clone G025H7) BioLegend 353716; RRID: AB_2561448 Mouse anti-human CXCR3 PE (clone G025H7) BioLegend 353706; RRID: AB_10962912 Mouse anti-human Erk1/2 (pT202/pY204) BV421 (clone 20A) BD Biosciences 562981; RRID: AB_2737930 Mouse anti-human GATA-3 PE-Cy7 (clone L50-823) BD Biosciences 560405; RRID: AB_1645544 Mouse anti-human IFNγ FITC (clone B27) BD Biosciences 552887; RRID: AB_394516 Mouse anti-human IFNγ Purified (clone B27) BD Biosciences 554698; RRID: AB_395516 Mouse anti-human IL-2 BV421 (clone MQ1-17H12) BD Biosciences 564164; RRID: AB_2738635 Mouse anti-human IL-4 Purified (clone MP4-25D2) BD Biosciences 554481; RRID: AB_395421 Mouse anti-human IL-4 BV711 (clone MP4-25D2) BD Biosciences 564112; RRID: AB_2738600 Mouse anti-human IL-17A BV786 (clone N49-653) BD Biosciences 563745; RRID: AB_2738401 Mouse anti-human IL-17F BV786 (clone O33-782) BD Biosciences 564265; RRID: AB_2869556 Mouse anti-human NF-kB (pS529) PE (clone K10-859.12.50) BD Biosciences 558423; RRID: AB_647222 Mouse anti-human NFATc3 (pS240) AF647 (clone C-3) Santa Cruz Biotechnology sc-365786 AF647; RRID: AB_10844623 Mouse anti-human RORγt BV421 (clone Q21-559) BD Biosciences 563282; RRID: AB_2738114 Mouse anti-human RORγt PE (clone Q21-559) BD Biosciences 563081; RRID: AB_2686896 Mouse anti-human RUNX3 BV421 (clone R3-5G4) BD Biosciences 565742; RRID: AB_2916369 Mouse anti-human SSEA4 APC (clone MC-813-70) BioLegend 330417; RRID: AB_2616818 Mouse anti-human T-bet AF647 (clone 4-B10) BD Biosciences 561264; RRID: AB_10563424 Mouse anti-human ThPOK PE (clone 11H11A14) BioLegend 656404; RRID: AB_2563009 Mouse anti-human TRA-1-81 PE (clone TRA-1-81) BioLegend 330708; RRID: AB_1089243 Mouse anti-human ZAP70 (pY319) / Syk (pY352) PE-Cy7 (clone 17A/P-ZAP70) BD Biosciences 561458; RRID: AB_10696417 TotalSeqTM-C Human Universal Cocktail, V1.0 BioLegend 399905; RRID: AB_2876728 Biological samples Human postnatal thymus from thymectomy (Donor ID: sex, age): T085: M, 8 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T087: F, 4 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T088: M, 4.5 months old BC Children’s Hospital N/A (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human Flt-3 Ligand/FLT3L Protein R&D Systems 308-FK Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems 291-G1 Recombinant Human IL-1β STEMCELL Technologies 78034.1 Recombinant Human IL-2 (Proleukin) Iovance Biotherapeutics 02130181 Recombinant Human IL-2 Protein STEMCELL Technologies 78036 Recombinant Human IL-3 Protein R&D Systems 203-IL Recombinant Human IL-4 Protein STEMCELL Technologies 78045 Recombinant Human IL-6 Protein STEMCELL Technologies 78148 Recombinant Human IL-6 Protein R&D Systems 206-IL Recombinant Human IL-7 Protein R&D Systems 207-IL Recombinant Human IL-11 Protein R&D Systems 218-IL Recombinant Human IL-12 (p70) BD Biosciences 554613 Recombinant Human IL-15 Protein R&D Systems 247-ILB Recombinant Human IL-18 Protein R&D Systems 9124-IL Recombinant Human IL-21 Protein R&D Systems 8879-IL Recombinant Human IL-23 Protein Thermo Fisher Scientific 200-23-10UG Recombinant Human Jagged 1 Fc Chimera Protein, CF R&D Systems 1277-JG Recombinant Human Jagged 2 Fc Chimera Protein, CF R&D Systems 1726-JG Recombinant Human SCF Protein R&D Systems 255-SC Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF R&D Systems 11409-BH-010 Recombinant Human Thrombopoietin (TPO, NS0-expressed) Protein R&D Systems 288-TPN Recombinant Human TNF-alpha Protein R&D Systems 210-TA Recombinant Human VEGF 165 Protein R&D Systems 293-VE Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF R&D Systems 643-VM-200 RetroNectin Takara Bio (Cedarlane) T100B(CB) ROCK Inhibitor Y-27632 STEMCELL Technologies 72308 SB 431542 hydrate Sigma-Aldrich S4317 Transferrin Sigma-Aldrich 10652202001 Z-VAD-FMK R&D Systems FMK001 Zombie UVTM Fixable Viability Kit BioLegend 423108 1-Thioglycerol Sigma-Aldrich M6145 2-Mercaptoethanol Sigma-Aldrich M3148 Critical commercial assays Absolutely RNA Nanoprep Kit Agilent 400753 CD34 MicroBead Kit, human Miltenyi Biotec 130-046-702 Cytofix/Cytoperm TM Fixation/ Permeabilization Kit BD Biosciences 554714 EasySepTM Human Naı̈ve CD4 + T Cell Isolation Kit STEMCELL Technologies 19555 EasySepTM Release Human CD3 Positive Selection Kit STEMCELL Technologies 17751 eBiosciences FoxP3 / Transcription Factor Staining Buffer Set Thermo Fisher Scientific 00-5523-00 RosetteSep Human CD4 + T Cell Enrichment Cocktail STEMCELL Technologies 15062 (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e5 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data CITE-seq iPS11-derived T cells at Maturation Day M14 in PSC2 media This Study Mendeley Data: https://doi.org/10.17632/ k3xtwnzpyn.1 CITE-seq + scTCR-seq iPS11-derived T cells at Expansion Day X11 in PSC4 media This Study Same as above Analysis code and generated outputs for Day M14 CITE-seq data This Study GitHub: https://github.com/ stemcellbioengineering/stankiewiczmichaels-M14; Zenodo: https://doi.org/10.

    Article Title: Targeted inhibition of ELANE expression using adenine base editing to treat severe congenital neutropenia
    Article Snippet: CD34 + cells were expanded for up to 7 days in CD34 + cell expansion medium (StemSpan SFEM II, STEMCELL Technologies, #09655) supplemented with 1% penicillin/streptomycin, 1% glutamine, and cytokines: 20 ng/mL IL-3 (BioLegend, #578006), 20 ng/mL IL-6 (R&D Systems, #7270-IL-100/CF), 20 ng/mL TPO (BioLegend, #763706), 50 ng/mL SCF (R&D Systems, #11010-SC-100), and 50 ng/mL FLT3L (BioLegend, #550606). .. CD34 + HSPCs from ELANE -CN patients or HDs were cultured for 7 days in differentiation medium 1 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and human recombinant cytokines: 5 ng/mL SCF [R&D Systems, #11010-SC-100], 5 ng/mL IL-3 [BioLegend, #578006], 5 ng/mL GM-CSF [PeproTech, #300-03], and 1 ng/mL G-CSF [Lenograstim, Granocyte, Chugai Pharma, PZN 7253193]) at a density of >2 × 10 5 cells/mL, followed by 7 days in differentiation medium 2 (RPMI supplemented with 10% FCS, 1% penicillin/streptomycin, and 1 ng/mL G-CSF [Lenograstim, Granocyte, Chugai Pharma, PZN 7253193]). ..

    Derivative Assay:

    Article Title: Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rhesus CD34+ antibody (Clone 12.8) Fred Hutchinson Cancer Research Center N/A CD34-PE antibody (Clone 563) BD Biosciences # 550761 RRID:AB_393871 Mouse anti-human fetal hemoglobin BD Biosciences # 551796 RRID:AB_394253 APC rat anti-mouse IgG1 (Clone X56) BD Biosciences # 550874 RRID:AB_398470 CD14-FITC (Clone M5E2) BD Bioscience # 555397 RRID:AB_395798 CD20-PE (Clone 2H7) BD Bioscience # 555623 RRID:AB_395989 CD34-APC (Clone 563) BD Bioscience # 561209 RRID:AB_10683161 CD45-V450 (Clone D058-1283) BD Bioscience # 561291 RRID:AB_10611576 CD71-PE (Clone DF1513) GeneTex # GTX43030 RRID:AB_11170434 CD45-FITC (Clone MB4-6D6) Miltenyi Biotech # 130-119-764 RRID:AB_2751825 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 CD45RA-VioBlue (Clone T6D11) Miltenyi Biotech # 130-113-360 RRID:AB_2726132 CD117-PE (Clone 104D2) BioLegend # 313204 RRID:AB_314983 Goat anti-human IgG-AF488 Jackson ImmunoResearch # 109-545-088 RRID:AB_2337838 CD45-APC (Non-competing MB4-6D6 clone) Miltenyi Biotech # 130-123-787 RRID:AB_2819522 CD3-BV605 (Clone SP34) BD Bioscience # 562994 RRID:AB_2737938 CD8-APC-Vio 770 (Clone BW135/80) Miltenyi Biotech # 130-113-155 RRID:AB_2725983 CD4-BV650 (Clone L200) BD Bioscience # 563737 RRID:AB_2687486 CD20-BV421 (Clone 2H7) BioLegend # 302330 RRID:AB_10965543 CD45RA-PerCP-Cy5.5 (Clone T6D11) Miltenyi Biotech # 130-113-359 RRID:AB_2751109 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 Anti-mouse IgM beads Miltenyi Biotech # 130-047-302 RRID:AB_244359 Biological samples Frozen healthy human umbilical cord derived CD34+ HSPCs AllCells N/A Frozen/fresh rhesus CD34+ HSPCs NIH N/A Cryopreserved bone marrow CD34+ HSPCs StemCell Technologies # 70002 Chemicals, peptides, and recombinant proteins 7-AAD BioLegend # 420403 Granulocyte-Colony Stimulating Factor (G-CSF) Amgen NDC 55513-0530-10 Plerixafor Sigma-Aldrich # 110078-46-1 Ficoll-Paque PLUS density gradient media GE Healthcare # 17144002 Human stem cell factor (SCF) R&D Systems # 255-SC/CF Human thrombopoietin (TPO) R&D Systems # 288-TP/CF Human recombinant human Flt3-ligand, (Flt3) R&D Systems # 308-TP/CF 33NLS SpCas9 Laboratory of Scot A. Wolfe N/A Interleukin-3 (IL-3) R&D Systems 203-IL-100/CF (Continued on next page) Cell Stem Cell 32, 209–226.e1–e8, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Erythropoietin (EPO) Amgen # 55513-144-10 Dexamethasone VETone # 501012 Depo-estradiol Pfizer NDC 00009-0271-01 Fetal bovine serum (FBS) ThermoFisher Scientific # 43803 FBS Gemini # 100-106 Insulin Lily NDC 00002-8215 Holo-transferrin Sigma Aldrich # T0665-1G Bovine serum albumin (BSA) Roche # 3116956001 TCEP Thermo Fisher Scientific # 20490 Hoechst 33342 Thermo Fisher Scientific # R37165 IL-6 R&D Systems # 206-IL-200/CF IL-2 NCI # Ro 23-6019 RetroNectin (Takara Bio) # T100B Busulfex PDL BioPharma NDC 59148-070-90 Hydroxyurea Bristol-Myers Squibb NDC 68084- 284-01 Critical commercial assays Gibco CTS Detachable Dynabeads CD3/CD28 Kit Thermo Fisher Scientific # A56992 CellTrace Far Red Cell Proliferation Kit Thermo Fisher Scientific # C34564 Deposited data Raw sequencing data This paper PRJNA1045609 Experimental models: Cell lines

    Cell Isolation:

    Article Title: Tunable differentiation of human CD4 + and CD8 + T cells from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-human CCR7 BV605 (clone 2-L1-A) BD Biosciences 566754; RRID: AB_2869850 Mouse anti-human CCR7 BUV661 (clone 2-L1-A) BD Biosciences 749824; RRID: AB_2874072 Mouse anti-human CXCR3 BV421 (clone G025H7) BioLegend 353716; RRID: AB_2561448 Mouse anti-human CXCR3 PE (clone G025H7) BioLegend 353706; RRID: AB_10962912 Mouse anti-human Erk1/2 (pT202/pY204) BV421 (clone 20A) BD Biosciences 562981; RRID: AB_2737930 Mouse anti-human GATA-3 PE-Cy7 (clone L50-823) BD Biosciences 560405; RRID: AB_1645544 Mouse anti-human IFNγ FITC (clone B27) BD Biosciences 552887; RRID: AB_394516 Mouse anti-human IFNγ Purified (clone B27) BD Biosciences 554698; RRID: AB_395516 Mouse anti-human IL-2 BV421 (clone MQ1-17H12) BD Biosciences 564164; RRID: AB_2738635 Mouse anti-human IL-4 Purified (clone MP4-25D2) BD Biosciences 554481; RRID: AB_395421 Mouse anti-human IL-4 BV711 (clone MP4-25D2) BD Biosciences 564112; RRID: AB_2738600 Mouse anti-human IL-17A BV786 (clone N49-653) BD Biosciences 563745; RRID: AB_2738401 Mouse anti-human IL-17F BV786 (clone O33-782) BD Biosciences 564265; RRID: AB_2869556 Mouse anti-human NF-kB (pS529) PE (clone K10-859.12.50) BD Biosciences 558423; RRID: AB_647222 Mouse anti-human NFATc3 (pS240) AF647 (clone C-3) Santa Cruz Biotechnology sc-365786 AF647; RRID: AB_10844623 Mouse anti-human RORγt BV421 (clone Q21-559) BD Biosciences 563282; RRID: AB_2738114 Mouse anti-human RORγt PE (clone Q21-559) BD Biosciences 563081; RRID: AB_2686896 Mouse anti-human RUNX3 BV421 (clone R3-5G4) BD Biosciences 565742; RRID: AB_2916369 Mouse anti-human SSEA4 APC (clone MC-813-70) BioLegend 330417; RRID: AB_2616818 Mouse anti-human T-bet AF647 (clone 4-B10) BD Biosciences 561264; RRID: AB_10563424 Mouse anti-human ThPOK PE (clone 11H11A14) BioLegend 656404; RRID: AB_2563009 Mouse anti-human TRA-1-81 PE (clone TRA-1-81) BioLegend 330708; RRID: AB_1089243 Mouse anti-human ZAP70 (pY319) / Syk (pY352) PE-Cy7 (clone 17A/P-ZAP70) BD Biosciences 561458; RRID: AB_10696417 TotalSeqTM-C Human Universal Cocktail, V1.0 BioLegend 399905; RRID: AB_2876728 Biological samples Human postnatal thymus from thymectomy (Donor ID: sex, age): T085: M, 8 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T087: F, 4 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T088: M, 4.5 months old BC Children’s Hospital N/A (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human Flt-3 Ligand/FLT3L Protein R&D Systems 308-FK Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems 291-G1 Recombinant Human IL-1β STEMCELL Technologies 78034.1 Recombinant Human IL-2 (Proleukin) Iovance Biotherapeutics 02130181 Recombinant Human IL-2 Protein STEMCELL Technologies 78036 Recombinant Human IL-3 Protein R&D Systems 203-IL Recombinant Human IL-4 Protein STEMCELL Technologies 78045 Recombinant Human IL-6 Protein STEMCELL Technologies 78148 Recombinant Human IL-6 Protein R&D Systems 206-IL Recombinant Human IL-7 Protein R&D Systems 207-IL Recombinant Human IL-11 Protein R&D Systems 218-IL Recombinant Human IL-12 (p70) BD Biosciences 554613 Recombinant Human IL-15 Protein R&D Systems 247-ILB Recombinant Human IL-18 Protein R&D Systems 9124-IL Recombinant Human IL-21 Protein R&D Systems 8879-IL Recombinant Human IL-23 Protein Thermo Fisher Scientific 200-23-10UG Recombinant Human Jagged 1 Fc Chimera Protein, CF R&D Systems 1277-JG Recombinant Human Jagged 2 Fc Chimera Protein, CF R&D Systems 1726-JG Recombinant Human SCF Protein R&D Systems 255-SC Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF R&D Systems 11409-BH-010 Recombinant Human Thrombopoietin (TPO, NS0-expressed) Protein R&D Systems 288-TPN Recombinant Human TNF-alpha Protein R&D Systems 210-TA Recombinant Human VEGF 165 Protein R&D Systems 293-VE Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF R&D Systems 643-VM-200 RetroNectin Takara Bio (Cedarlane) T100B(CB) ROCK Inhibitor Y-27632 STEMCELL Technologies 72308 SB 431542 hydrate Sigma-Aldrich S4317 Transferrin Sigma-Aldrich 10652202001 Z-VAD-FMK R&D Systems FMK001 Zombie UVTM Fixable Viability Kit BioLegend 423108 1-Thioglycerol Sigma-Aldrich M6145 2-Mercaptoethanol Sigma-Aldrich M3148 Critical commercial assays Absolutely RNA Nanoprep Kit Agilent 400753 CD34 MicroBead Kit, human Miltenyi Biotec 130-046-702 Cytofix/Cytoperm TM Fixation/ Permeabilization Kit BD Biosciences 554714 EasySepTM Human Naı̈ve CD4 + T Cell Isolation Kit STEMCELL Technologies 19555 EasySepTM Release Human CD3 Positive Selection Kit STEMCELL Technologies 17751 eBiosciences FoxP3 / Transcription Factor Staining Buffer Set Thermo Fisher Scientific 00-5523-00 RosetteSep Human CD4 + T Cell Enrichment Cocktail STEMCELL Technologies 15062 (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e5 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data CITE-seq iPS11-derived T cells at Maturation Day M14 in PSC2 media This Study Mendeley Data: https://doi.org/10.17632/ k3xtwnzpyn.1 CITE-seq + scTCR-seq iPS11-derived T cells at Expansion Day X11 in PSC4 media This Study Same as above Analysis code and generated outputs for Day M14 CITE-seq data This Study GitHub: https://github.com/ stemcellbioengineering/stankiewiczmichaels-M14; Zenodo: https://doi.org/10.

    Selection:

    Article Title: Tunable differentiation of human CD4 + and CD8 + T cells from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-human CCR7 BV605 (clone 2-L1-A) BD Biosciences 566754; RRID: AB_2869850 Mouse anti-human CCR7 BUV661 (clone 2-L1-A) BD Biosciences 749824; RRID: AB_2874072 Mouse anti-human CXCR3 BV421 (clone G025H7) BioLegend 353716; RRID: AB_2561448 Mouse anti-human CXCR3 PE (clone G025H7) BioLegend 353706; RRID: AB_10962912 Mouse anti-human Erk1/2 (pT202/pY204) BV421 (clone 20A) BD Biosciences 562981; RRID: AB_2737930 Mouse anti-human GATA-3 PE-Cy7 (clone L50-823) BD Biosciences 560405; RRID: AB_1645544 Mouse anti-human IFNγ FITC (clone B27) BD Biosciences 552887; RRID: AB_394516 Mouse anti-human IFNγ Purified (clone B27) BD Biosciences 554698; RRID: AB_395516 Mouse anti-human IL-2 BV421 (clone MQ1-17H12) BD Biosciences 564164; RRID: AB_2738635 Mouse anti-human IL-4 Purified (clone MP4-25D2) BD Biosciences 554481; RRID: AB_395421 Mouse anti-human IL-4 BV711 (clone MP4-25D2) BD Biosciences 564112; RRID: AB_2738600 Mouse anti-human IL-17A BV786 (clone N49-653) BD Biosciences 563745; RRID: AB_2738401 Mouse anti-human IL-17F BV786 (clone O33-782) BD Biosciences 564265; RRID: AB_2869556 Mouse anti-human NF-kB (pS529) PE (clone K10-859.12.50) BD Biosciences 558423; RRID: AB_647222 Mouse anti-human NFATc3 (pS240) AF647 (clone C-3) Santa Cruz Biotechnology sc-365786 AF647; RRID: AB_10844623 Mouse anti-human RORγt BV421 (clone Q21-559) BD Biosciences 563282; RRID: AB_2738114 Mouse anti-human RORγt PE (clone Q21-559) BD Biosciences 563081; RRID: AB_2686896 Mouse anti-human RUNX3 BV421 (clone R3-5G4) BD Biosciences 565742; RRID: AB_2916369 Mouse anti-human SSEA4 APC (clone MC-813-70) BioLegend 330417; RRID: AB_2616818 Mouse anti-human T-bet AF647 (clone 4-B10) BD Biosciences 561264; RRID: AB_10563424 Mouse anti-human ThPOK PE (clone 11H11A14) BioLegend 656404; RRID: AB_2563009 Mouse anti-human TRA-1-81 PE (clone TRA-1-81) BioLegend 330708; RRID: AB_1089243 Mouse anti-human ZAP70 (pY319) / Syk (pY352) PE-Cy7 (clone 17A/P-ZAP70) BD Biosciences 561458; RRID: AB_10696417 TotalSeqTM-C Human Universal Cocktail, V1.0 BioLegend 399905; RRID: AB_2876728 Biological samples Human postnatal thymus from thymectomy (Donor ID: sex, age): T085: M, 8 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T087: F, 4 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T088: M, 4.5 months old BC Children’s Hospital N/A (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human Flt-3 Ligand/FLT3L Protein R&D Systems 308-FK Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems 291-G1 Recombinant Human IL-1β STEMCELL Technologies 78034.1 Recombinant Human IL-2 (Proleukin) Iovance Biotherapeutics 02130181 Recombinant Human IL-2 Protein STEMCELL Technologies 78036 Recombinant Human IL-3 Protein R&D Systems 203-IL Recombinant Human IL-4 Protein STEMCELL Technologies 78045 Recombinant Human IL-6 Protein STEMCELL Technologies 78148 Recombinant Human IL-6 Protein R&D Systems 206-IL Recombinant Human IL-7 Protein R&D Systems 207-IL Recombinant Human IL-11 Protein R&D Systems 218-IL Recombinant Human IL-12 (p70) BD Biosciences 554613 Recombinant Human IL-15 Protein R&D Systems 247-ILB Recombinant Human IL-18 Protein R&D Systems 9124-IL Recombinant Human IL-21 Protein R&D Systems 8879-IL Recombinant Human IL-23 Protein Thermo Fisher Scientific 200-23-10UG Recombinant Human Jagged 1 Fc Chimera Protein, CF R&D Systems 1277-JG Recombinant Human Jagged 2 Fc Chimera Protein, CF R&D Systems 1726-JG Recombinant Human SCF Protein R&D Systems 255-SC Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF R&D Systems 11409-BH-010 Recombinant Human Thrombopoietin (TPO, NS0-expressed) Protein R&D Systems 288-TPN Recombinant Human TNF-alpha Protein R&D Systems 210-TA Recombinant Human VEGF 165 Protein R&D Systems 293-VE Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF R&D Systems 643-VM-200 RetroNectin Takara Bio (Cedarlane) T100B(CB) ROCK Inhibitor Y-27632 STEMCELL Technologies 72308 SB 431542 hydrate Sigma-Aldrich S4317 Transferrin Sigma-Aldrich 10652202001 Z-VAD-FMK R&D Systems FMK001 Zombie UVTM Fixable Viability Kit BioLegend 423108 1-Thioglycerol Sigma-Aldrich M6145 2-Mercaptoethanol Sigma-Aldrich M3148 Critical commercial assays Absolutely RNA Nanoprep Kit Agilent 400753 CD34 MicroBead Kit, human Miltenyi Biotec 130-046-702 Cytofix/Cytoperm TM Fixation/ Permeabilization Kit BD Biosciences 554714 EasySepTM Human Naı̈ve CD4 + T Cell Isolation Kit STEMCELL Technologies 19555 EasySepTM Release Human CD3 Positive Selection Kit STEMCELL Technologies 17751 eBiosciences FoxP3 / Transcription Factor Staining Buffer Set Thermo Fisher Scientific 00-5523-00 RosetteSep Human CD4 + T Cell Enrichment Cocktail STEMCELL Technologies 15062 (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e5 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data CITE-seq iPS11-derived T cells at Maturation Day M14 in PSC2 media This Study Mendeley Data: https://doi.org/10.17632/ k3xtwnzpyn.1 CITE-seq + scTCR-seq iPS11-derived T cells at Expansion Day X11 in PSC4 media This Study Same as above Analysis code and generated outputs for Day M14 CITE-seq data This Study GitHub: https://github.com/ stemcellbioengineering/stankiewiczmichaels-M14; Zenodo: https://doi.org/10.

    Staining:

    Article Title: Tunable differentiation of human CD4 + and CD8 + T cells from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse anti-human CCR7 BV605 (clone 2-L1-A) BD Biosciences 566754; RRID: AB_2869850 Mouse anti-human CCR7 BUV661 (clone 2-L1-A) BD Biosciences 749824; RRID: AB_2874072 Mouse anti-human CXCR3 BV421 (clone G025H7) BioLegend 353716; RRID: AB_2561448 Mouse anti-human CXCR3 PE (clone G025H7) BioLegend 353706; RRID: AB_10962912 Mouse anti-human Erk1/2 (pT202/pY204) BV421 (clone 20A) BD Biosciences 562981; RRID: AB_2737930 Mouse anti-human GATA-3 PE-Cy7 (clone L50-823) BD Biosciences 560405; RRID: AB_1645544 Mouse anti-human IFNγ FITC (clone B27) BD Biosciences 552887; RRID: AB_394516 Mouse anti-human IFNγ Purified (clone B27) BD Biosciences 554698; RRID: AB_395516 Mouse anti-human IL-2 BV421 (clone MQ1-17H12) BD Biosciences 564164; RRID: AB_2738635 Mouse anti-human IL-4 Purified (clone MP4-25D2) BD Biosciences 554481; RRID: AB_395421 Mouse anti-human IL-4 BV711 (clone MP4-25D2) BD Biosciences 564112; RRID: AB_2738600 Mouse anti-human IL-17A BV786 (clone N49-653) BD Biosciences 563745; RRID: AB_2738401 Mouse anti-human IL-17F BV786 (clone O33-782) BD Biosciences 564265; RRID: AB_2869556 Mouse anti-human NF-kB (pS529) PE (clone K10-859.12.50) BD Biosciences 558423; RRID: AB_647222 Mouse anti-human NFATc3 (pS240) AF647 (clone C-3) Santa Cruz Biotechnology sc-365786 AF647; RRID: AB_10844623 Mouse anti-human RORγt BV421 (clone Q21-559) BD Biosciences 563282; RRID: AB_2738114 Mouse anti-human RORγt PE (clone Q21-559) BD Biosciences 563081; RRID: AB_2686896 Mouse anti-human RUNX3 BV421 (clone R3-5G4) BD Biosciences 565742; RRID: AB_2916369 Mouse anti-human SSEA4 APC (clone MC-813-70) BioLegend 330417; RRID: AB_2616818 Mouse anti-human T-bet AF647 (clone 4-B10) BD Biosciences 561264; RRID: AB_10563424 Mouse anti-human ThPOK PE (clone 11H11A14) BioLegend 656404; RRID: AB_2563009 Mouse anti-human TRA-1-81 PE (clone TRA-1-81) BioLegend 330708; RRID: AB_1089243 Mouse anti-human ZAP70 (pY319) / Syk (pY352) PE-Cy7 (clone 17A/P-ZAP70) BD Biosciences 561458; RRID: AB_10696417 TotalSeqTM-C Human Universal Cocktail, V1.0 BioLegend 399905; RRID: AB_2876728 Biological samples Human postnatal thymus from thymectomy (Donor ID: sex, age): T085: M, 8 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T087: F, 4 months old BC Children’s Hospital N/A Human postnatal thymus from thymectomy (Donor ID: sex, age): T088: M, 4.5 months old BC Children’s Hospital N/A (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Human Flt-3 Ligand/FLT3L Protein R&D Systems 308-FK Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems 291-G1 Recombinant Human IL-1β STEMCELL Technologies 78034.1 Recombinant Human IL-2 (Proleukin) Iovance Biotherapeutics 02130181 Recombinant Human IL-2 Protein STEMCELL Technologies 78036 Recombinant Human IL-3 Protein R&D Systems 203-IL Recombinant Human IL-4 Protein STEMCELL Technologies 78045 Recombinant Human IL-6 Protein STEMCELL Technologies 78148 Recombinant Human IL-6 Protein R&D Systems 206-IL Recombinant Human IL-7 Protein R&D Systems 207-IL Recombinant Human IL-11 Protein R&D Systems 218-IL Recombinant Human IL-12 (p70) BD Biosciences 554613 Recombinant Human IL-15 Protein R&D Systems 247-ILB Recombinant Human IL-18 Protein R&D Systems 9124-IL Recombinant Human IL-21 Protein R&D Systems 8879-IL Recombinant Human IL-23 Protein Thermo Fisher Scientific 200-23-10UG Recombinant Human Jagged 1 Fc Chimera Protein, CF R&D Systems 1277-JG Recombinant Human Jagged 2 Fc Chimera Protein, CF R&D Systems 1726-JG Recombinant Human SCF Protein R&D Systems 255-SC Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF R&D Systems 11409-BH-010 Recombinant Human Thrombopoietin (TPO, NS0-expressed) Protein R&D Systems 288-TPN Recombinant Human TNF-alpha Protein R&D Systems 210-TA Recombinant Human VEGF 165 Protein R&D Systems 293-VE Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF R&D Systems 643-VM-200 RetroNectin Takara Bio (Cedarlane) T100B(CB) ROCK Inhibitor Y-27632 STEMCELL Technologies 72308 SB 431542 hydrate Sigma-Aldrich S4317 Transferrin Sigma-Aldrich 10652202001 Z-VAD-FMK R&D Systems FMK001 Zombie UVTM Fixable Viability Kit BioLegend 423108 1-Thioglycerol Sigma-Aldrich M6145 2-Mercaptoethanol Sigma-Aldrich M3148 Critical commercial assays Absolutely RNA Nanoprep Kit Agilent 400753 CD34 MicroBead Kit, human Miltenyi Biotec 130-046-702 Cytofix/Cytoperm TM Fixation/ Permeabilization Kit BD Biosciences 554714 EasySepTM Human Naı̈ve CD4 + T Cell Isolation Kit STEMCELL Technologies 19555 EasySepTM Release Human CD3 Positive Selection Kit STEMCELL Technologies 17751 eBiosciences FoxP3 / Transcription Factor Staining Buffer Set Thermo Fisher Scientific 00-5523-00 RosetteSep Human CD4 + T Cell Enrichment Cocktail STEMCELL Technologies 15062 (Continued on next page) Cell Stem Cell 33, 73–90.e1–e14, January 8, 2026 e5 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data CITE-seq iPS11-derived T cells at Maturation Day M14 in PSC2 media This Study Mendeley Data: https://doi.org/10.17632/ k3xtwnzpyn.1 CITE-seq + scTCR-seq iPS11-derived T cells at Expansion Day X11 in PSC4 media This Study Same as above Analysis code and generated outputs for Day M14 CITE-seq data This Study GitHub: https://github.com/ stemcellbioengineering/stankiewiczmichaels-M14; Zenodo: https://doi.org/10.

    In Vitro:

    Article Title: Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE.
    Article Snippet: CD34+ cells were enriched by immunomagnetic bead selection using a CliniMACS Plus or AutoMACS instrument (Miltenyi Biotec). .. Human CD34+ cells were cultured in vitro using complete medium (CM) consisting of StemSpan II (StemCell Technologies) medium supplemented with 100 ng μl−1 human stem cell factor (R&D systems, 255-SC/CF), 100 ng μl−1 human thrombopoietin (R&D systems, 288-TP/CF) and 100 ng μl−1 human FLT-3 ligand (R&D systems, 308-FK/CF). ..



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